How to

Replace the

Glass

Chromatographic Tube

in

Pharmacia / GE / Cytiva

XK 16 and XK 26

Columns

Step 1

To change the XK 16 or XK 26 glass chromatographic tube please consider soap, gloves and the dismantling tool. The gloves can protect your hands from broken glass. If the existing glass chromatographic tube is broken a blotter on your work surface could help contain small pieces of glass when you take the column apart.

These instructions have not been reviewed, tested or approved by either Cytiva or GE or Pharmacia. Use these instructions at your own risk.

Cytiva, GE and Pharmacia are trademarks or registered trademarks of their respective companies.

Step 2

Loosen the black adjusting knob by turning it towards you.

This loosens the gasket that is pressed against the glass chromatographic tube for a good seal.

Step 3

Remove the bottom piece from the end piece by turning it towards you.

Step 4

The bottom piece removed from the end piece.

Step 5

Using the dismantling tool remove the threaded, circular plastic fitting by turning the tool towards you.

Step 6

When the threaded, circular plastic fitting is removed, the white washer shown in the photo may fall out.

Step 7

Remove the end piece by gently turning the end piece towards you and sliding it off the glass chromatographic tube.

Step 8

The end piece is removed from the thermostatic jacket and the glass chromatographic tube.

Step 9

Please now repeat the process on the other end of the column.

Loosen the black adjusting knob by turning it towards you. This loosens the gasket that is pressed against the glass chromatographic tube for a good seal.

Step 10

Remove the bottom piece from the end piece by turning it towards you.

Step 11

The bottom piece and the plunger removed from the end piece.

Step 12

Using the dismantling tool, remove the threaded, circular plastic fitting by turning the tool towards you.

Step 13

When the threaded, circular plastic fitting is removed, the white washer shown in the photo may fall out.

Step 14

Remove the end piece by gently turning the end piece towards you and sliding it off the glass chromatographic tube.

Step 15

Now you are ready to replace the broken glass tube with the new replacement and reassemble the column.

Please safely discard the broken glass chromatographic tube.

Step 16

Rub a small amount of soap all the way around the outside end of the replacement glass chromatographic tube.

Step 17

Rub a small amount of soap all the way around the inside of the black gasket inside the end piece.

Step 18

Slowly and gently slide the replacement glass chromatographic tube into the end piece and then screw the thermostatic jacket into the end piece.

Step 19

Using the dismantling tool, screw the threaded, circular plastic fitting into the end piece by turning the tool away from you.

Please be sure the white washer is in place.

Step 20

Shows how the replacement glass chromatographic tube should look when the circular plastic fitting is tightened.

If the replacement glass chromatographic tube protrudes further from the circular plastic fitting than shown in the photo the fit may not be correct on the other end of the column.

Step 21

Now insert the plunger assembly.

Step 22

Screw the bottom piece into the end piece by turning it away from you.

Step 23

Tighten the black adjusting knob by turning it away from you.

Step 24

Slide the thermostatic jacket onto the replacement glass chromatographic tube and screw it into the end piece.

Step 25

Please now repeat the process on the other end of the column.

Rub a small amount of soap all the way around the outside of the replacement glass chromatographic tube.

Rub a small amount of soap all the way around the inside of the black gasket inside the end piece.

Step 26

Slowly and gently slide the replacement glass chromatographic tube into the end piece and then screw the thermostatic jacket into the end piece.

Step 27

Using the dismantling tool, screw the threaded, circular plastic fitting into the end piece by turning the tool away from you.

Please be sure the white washer is in place.

Step 28

Screw the bottom piece into the end piece by turning it away from you.

Then tighten the black adjusting knob by turning it away from you.

Step 29



Congratulations !



You have just completed installing a replacement glass chromatographic tube in your column.